2D HSQC NMR analysis of plant cell wall material in DMSO-d6/HMPA-d18

The cell wall material gel is the product of the cell wall material directly formed with deuterated solvent in the nuclear magnetic resonance tube. The well-resolved/dispersed 2D 13C-1H related nuclear magnetic resonance spectra (2D HSQC NMR)can be obtained without the separation of components. Deuterated dimethyl sulfoxide (DMSO-d6), deuterated dimethyl sulfoxide/deuterated pyridine (DMSO-d6/pyridine-d5) and deuterated dimethyl sulfoxide/deuterated hexamethylphosphoryltriamine (DMSO-d6/HMPA-d18) were selected to dissolve the cell wall of poplar (angiosperm). The spectrum signals under DMSO-d6/HMPA-d18 were the most abundant, and the correlation of p-hydroxyphenyl signals could be easily obtained. Therefore, DMSO-d6/ HMPA-d18 improves the resolution and intensity of the spectrum. DMSO-d6/HMPA-d18 was used to characterize the two-dimensional NMR structure of pine (gymnosperms), and the spectrum showed high-resolution polysaccharide correlation and lignin structure. Studies have shown that this method is suitable for the detection of cell wall substances in plants.Therefore, the 2D HSQC NMR study under DMSO-d6/HMPA-d18 is a faster and greener method to evaluate the structure of plant cell wall.