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Separation and purification to prepare N-acetylneuraminic acid after oxalic acid hydrolysis of polysialic acid

  • 1.

    Institute of plasma physics, Chinese Academy of Sciences(ASIPP), Hefei 230031,China

  • 2.

    Huainan new energy research center, Hefei 232001,China

  • 3.

    University of Science and Technology of China, Hefei, 230026,China

Cite this: JUSTC, 2020, 50(5): 605-611
https://doi.org/10.3969/j.issn.0253-2778.2020.05.008
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  • Received Date: April 15, 2019
  • Revised Date: November 24, 2019
  • Accepted Date: November 24, 2019
  • Published Date: May 30, 2020
    Abstract
  • The polysialic acid was obtained from fermentation of escherichia coli, and hydrolyzed to separate, purify and prepare the N-acetylneuraminic acid. In order to study the process conditions of separation and purification to prepare high purity of n-acetylneuraminic acid from the polysialic acid which was hydrolyzed by the oxalic acid, so as to simplify the process and reduce the production cost, the authors investigated emphatically the hydrolysis of the polysialic acid by oxalic acid , contrasted firstly the effects on hydrolysis of polysialic acids between oxalic acids as other conventional hydrolytic acids and determined oxalic acids as hydrolytic acids. The best operating parameters of the oxalic acid hydrolysis of the polysialic acid was determined by the orthogonal experiment: hydrolysis temperature 90 ℃, hydrolysis time 3.5 h, hydrolysis acid concentration 0.1 mol/L and diluted multiples of polysialic acid 5 times. In these conditions, the hydrolyze rate of the polysialic acid was more than 94 %, and the hydrolyze yield was more than 97 %. The separation and purification conditions were optimized through experiments, and the separation and purification process for the preparation of high purity N-acetylneuraminic acid was obtained. In these process conditions, the analytic results of the infrared spectrum and HPLC showed that the purity of the N-acetylneuraminic acid product was 98.5 %, and the yield was 58.4 %.
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    2020 No. 5 Volume 50 Issue 5 PP. 605-611
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