ISSN 0253-2778

CN 34-1054/N

Open AccessOpen Access JUSTC

Detection of caspase-3 by fluorescence quenching effect of a self-assembly nanoparticle

Cite this:
https://doi.org/10.3969/j.issn.0253-2778.2015.08.005
  • Received Date: 20 April 2015
  • Rev Recd Date: 23 May 2015
  • Publish Date: 31 August 2015
  • Caspase-3 is a very important protease in cell apoptosis and plays a crucial role in many diseases. A new probe Ac-Asp-Glu-Val-Asp-Cys(StBu)-Lys(FITC)-CBT (1) was reported, which can be reduced by TCEP and cleaved by caspase-3 to yield the amphiphilic dimer (1-D).1-D can quickly self-assemble into nanoparticles to induce a clear fluorescence quenching effect because of the aggregation-caused quench. The nanoparticles were characterized by means of dynamic light scattering (DLS), transmission electron microscope (TEM), and high performance liquid chromatography (HPLC). As a result, the fluorescence intensity has a linear correlation with the concentration of caspase-3, which could be applied to sensing caspase-3 activity in vivo in the future.
    Caspase-3 is a very important protease in cell apoptosis and plays a crucial role in many diseases. A new probe Ac-Asp-Glu-Val-Asp-Cys(StBu)-Lys(FITC)-CBT (1) was reported, which can be reduced by TCEP and cleaved by caspase-3 to yield the amphiphilic dimer (1-D).1-D can quickly self-assemble into nanoparticles to induce a clear fluorescence quenching effect because of the aggregation-caused quench. The nanoparticles were characterized by means of dynamic light scattering (DLS), transmission electron microscope (TEM), and high performance liquid chromatography (HPLC). As a result, the fluorescence intensity has a linear correlation with the concentration of caspase-3, which could be applied to sensing caspase-3 activity in vivo in the future.
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