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卵母细胞的低温保存:历史,成就和未来

Cryopreservation of oocytes: history, achievements and future

  • 摘要: 近年来由于肿瘤和非肿瘤因素的影响,对女性生育力保存的需求越来越高,满足这些需求将会是未来几年的一个热门话题。卵母细胞的冷冻保存是实现女性生育力保存的一个可行的选择,在哺乳动物基因库和人类卵母细胞库方面已经取得了重大进展。本文将系统介绍卵母细胞低温保存的研究历史和玻璃化保存技术,重点介绍保存中使用的玻璃化载体。此外,我们还总结了卵母细胞玻璃化保存的基本原理,讨论了玻璃化对于卵母细胞质量的影响,并且提出了改善卵母细胞低温保存效率的一些策略。最后,我们对卵母细胞低温保存技术进行了总结,并对其发展前景进行了展望。

     

    Abstract: There have been increasing requirements for women’s fertility preservation due to oncological and nononcological reasons in recent years, and meeting these demands will be a hot topic in the coming years. Oocyte cryopreservation is a workable option for preserving women’s fertility, and great advances have already been made and much progress has been made in mammalian gene banking and human oocyte banks. In this paper, we systematically introduce the history of oocyte cryopreservation and vitrification technology and highlight the vitrification carrier. Furthermore, we summarize the fundamentals of oocyte vitrification and discuss the effects of vitrification on oocyte quality. Strategies to improve the effect of oocyte cryopreservation are also proposed. At the end of this review, we conclude oocyte cryopreservation and outline future perspectives.

     

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