ISSN 0253-2778

CN 34-1054/N

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Open AccessOpen Access JUSTC Original Paper

Transdermal delivery peptide promoted the regulation of mice hair growth by epidermal growth factor

  • 1.

    Department of Laboratory Medicine, The First Affiliated Hospital of USTC (Anhui Provincial Hospital), Hefei 230036, China

  • 2.

    Reproductive Medicine Center, The First Affiliated Hospital of USTC (Anhui Provincial Hospital), Hefei 230036, China

  • 3.

    The First Affiliated Hospital of USTC (Anhui Provincial Hospital),Anhui Provincial Stereotactic Neurosurgical Institute, Hefei 230036, China

Cite this:
https://doi.org/10.3969/j.issn.0253-2778.2018.11.011
  • Received Date: 20 September 2018
  • Accepted Date: 20 October 2018
  • Rev Recd Date: 20 October 2018
  • Publish Date: 30 November 2018
    Abstract

  • Abstract

    TD1 was known as a peptide chaperon with 11 amino acids (ACSSSPSKHCG) that can facilitate transdermal delivery of proteins. Previous studies showed the maximum transdermal efficiency of fusion protein TD1-hEGF（T-E） was 16 folds higher than that of native hEGF. The second-generation of TD1-hEGF fused protein: TD1-hEGF-TD1(T-E-T) and TD1-TD1-hEGF (2T-E), designed based on the fact that transdermal efficiency was concentration-dependent of TD1, possessed considerable higher transdermal abilities than TD1-hEGF. To further detect the bioactivity of transdermal hEGF mediated by TD1, a hair growth experiments in mice was conducted. It was found that all the three fused proteins above can induce the transition of hair follicles from the telogen phase to the anagen phase of the hair cycle. These findings provide convincing evidences for improved practicability of the transdermal delivery peptide TD1 in cosmetic.

    Abstract

    TD1 was known as a peptide chaperon with 11 amino acids (ACSSSPSKHCG) that can facilitate transdermal delivery of proteins. Previous studies showed the maximum transdermal efficiency of fusion protein TD1-hEGF（T-E） was 16 folds higher than that of native hEGF. The second-generation of TD1-hEGF fused protein: TD1-hEGF-TD1(T-E-T) and TD1-TD1-hEGF (2T-E), designed based on the fact that transdermal efficiency was concentration-dependent of TD1, possessed considerable higher transdermal abilities than TD1-hEGF. To further detect the bioactivity of transdermal hEGF mediated by TD1, a hair growth experiments in mice was conducted. It was found that all the three fused proteins above can induce the transition of hair follicles from the telogen phase to the anagen phase of the hair cycle. These findings provide convincing evidences for improved practicability of the transdermal delivery peptide TD1 in cosmetic.
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    • References(20)
  • [1]
    KURMI B D, TEKCHANDANI P, PALIWAL R, et al. Transdermal drug delivery: Opportunities and challenges for controlled delivery of therapeutic agents using nanocarriers [J]. Current Drug Metabolism, 2017, 18: 481-95.
    [2]
    IBRAHIM Z A, HASSAN G F, ELGENDY H Y, et al. Evaluation of the efficacy of transdermal drug delivery of calcipotriol plus betamethasone versus tacrolimus in the treatment of vitiligo [J]. Journal of Cosmetic Dermatology, 2018: DOI=10.1111/jocd.12704.
    [3]
    AMMAR H O, GHORAB M, EL-NAHHAS S A, et al. Design of a transdermal delivery system for aspirin as an antithrombotic drug [J]. International Journal of Pharmaceutics, 2006, 327: 81-88.
    [4]
    SIXEL-DORING F, TRENKWALDER C. Rotigotine transdermal delivery for the treatment of restless legs syndrome [J]. Expert Opinionon Pharmacotherapy, 2010,11: 649-656.
    [5]
    JIANG T, WANG T, LI T, et al. Enhanced transdermal drug delivery by transfersome-embedded oligopeptide hydrogel for topical chemotherapy of melanoma [J]. ACS NANO, 2018, 12 (10): 9693-9701.
    [6]
    BROWN M B, MARTIN G P, JONES S A, et al. Dermal and transdermal drug delivery systems: Current and future prospects [J]. Drug Delivery, 2006, 13: 175-187.
    [7]
    COURTENAY A J, MCCRUDDEN M T C, MCAVOY K J, et al. Microneedle-mediated transdermal delivery of bevacizumab[J]. Molecular Pharmaceutics [J]. 2018,15: 3545-3556.
    [8]
    CEFALU W T. Concept, strategies, and feasibility of noninvasive insulin delivery [J]. Diabetes Care, 2004, 27: 239-246.
    [9]
    CHEN Y, SHEN Y, GUO X, et al. Transdermal protein delivery by a coadministered peptide identified via phage display [J]. Nature Biotechnology, 2006, 24: 455-460.
    [10]
    RUAN R Q, WANG S S, WANG C L, et al. Transdermal delivery of human epidermal growth factor facilitated by a peptide chaperon [J]. European Journal of Medicinal Chemistry, 2013, 62: 405-409.
    [11]
    JIN P P, LI F F, RUAN R Q, et al. Enhanced transdermal delivery of epidermal growth factor facilitated by dual peptide chaperone motifs [J]. Protein and Peptide Letters, 2014, 21: 550-555.
    [12]
    HERMAN A, HERMAN A P. Mechanism of action of herbs and their active constituents used in hair loss treatment [J]. Fitoterapia, 2016, 114: 18-25.
    [13]
    WU Z, SUN L, LIU G, et al. Hair follicle development and related gene and protein expression of skins in Rex rabbits during the first 8 weeks of life [J]. Asian-Australasian Journal of Animal Sciences, 2018: DOI= 10.5713/ajas.18.0256.
    [14]
    BICHSEL K J, GOGIA N, MALOUFF T, et al. Role for the epidermal growth factor receptor in chemotherapy-induced alopecia [J]. PLoS ONE, 2013, 8(7): e69368.
    [15]
    PAIK S H, YOON J S, RYU H H, et al. Pretreatment of epidermal growth factor promotes primary hair recovery via the dystrophic anagen pathway after chemotherapy-induced alopecia[J]. Experimental Dermatology, 2013, 22(7): 496-499.
    [16]
    WEE P, WANG Z. Epidermal growth factor receptor cell proliferation signaling pathways [J]. Cancers, 2017, 9(5): 52.
    [17]
    HUYNH E, LI J. Generation of Lactococcus lactis capable of coexpressing epidermal growth factor and trefoil factor to enhance in vitro wound healing [J]. Applied Microbiology and Biotechnology, 2015, 99(11): 4667-4677.
    [18]
    WALTER M N, WRIGHT K T, FULLER H R, et al. Mesenchymal stem cell-conditioned medium accelerates skin wound healing: An in vitro study of fibroblast and keratinocyte scratch assays [J]. Experimental Cell Research, 2010, 316(7): 1271-1281.
    [19]
    LUSK J B, LAM V Y, TOLWINSKI N S. Epidermal growth factor pathway signaling in drosophila embryogenesis: Tools for understanding cancer [J]. Cancers, 2017, 9(2): 16.
    [20]
    XING X J, YANG L, YOU Y, et al. Study of the biological function and penetration pathways of the mouse epidermal growth factor ethosomal delivery system [J]. Experimental Dermatology, 2011, 20(11): 945-947.
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    [1]
    KURMI B D, TEKCHANDANI P, PALIWAL R, et al. Transdermal drug delivery: Opportunities and challenges for controlled delivery of therapeutic agents using nanocarriers [J]. Current Drug Metabolism, 2017, 18: 481-95.
    [2]
    IBRAHIM Z A, HASSAN G F, ELGENDY H Y, et al. Evaluation of the efficacy of transdermal drug delivery of calcipotriol plus betamethasone versus tacrolimus in the treatment of vitiligo [J]. Journal of Cosmetic Dermatology, 2018: DOI=10.1111/jocd.12704.
    [3]
    AMMAR H O, GHORAB M, EL-NAHHAS S A, et al. Design of a transdermal delivery system for aspirin as an antithrombotic drug [J]. International Journal of Pharmaceutics, 2006, 327: 81-88.
    [4]
    SIXEL-DORING F, TRENKWALDER C. Rotigotine transdermal delivery for the treatment of restless legs syndrome [J]. Expert Opinionon Pharmacotherapy, 2010,11: 649-656.
    [5]
    JIANG T, WANG T, LI T, et al. Enhanced transdermal drug delivery by transfersome-embedded oligopeptide hydrogel for topical chemotherapy of melanoma [J]. ACS NANO, 2018, 12 (10): 9693-9701.
    [6]
    BROWN M B, MARTIN G P, JONES S A, et al. Dermal and transdermal drug delivery systems: Current and future prospects [J]. Drug Delivery, 2006, 13: 175-187.
    [7]
    COURTENAY A J, MCCRUDDEN M T C, MCAVOY K J, et al. Microneedle-mediated transdermal delivery of bevacizumab[J]. Molecular Pharmaceutics [J]. 2018,15: 3545-3556.
    [8]
    CEFALU W T. Concept, strategies, and feasibility of noninvasive insulin delivery [J]. Diabetes Care, 2004, 27: 239-246.
    [9]
    CHEN Y, SHEN Y, GUO X, et al. Transdermal protein delivery by a coadministered peptide identified via phage display [J]. Nature Biotechnology, 2006, 24: 455-460.
    [10]
    RUAN R Q, WANG S S, WANG C L, et al. Transdermal delivery of human epidermal growth factor facilitated by a peptide chaperon [J]. European Journal of Medicinal Chemistry, 2013, 62: 405-409.
    [11]
    JIN P P, LI F F, RUAN R Q, et al. Enhanced transdermal delivery of epidermal growth factor facilitated by dual peptide chaperone motifs [J]. Protein and Peptide Letters, 2014, 21: 550-555.
    [12]
    HERMAN A, HERMAN A P. Mechanism of action of herbs and their active constituents used in hair loss treatment [J]. Fitoterapia, 2016, 114: 18-25.
    [13]
    WU Z, SUN L, LIU G, et al. Hair follicle development and related gene and protein expression of skins in Rex rabbits during the first 8 weeks of life [J]. Asian-Australasian Journal of Animal Sciences, 2018: DOI= 10.5713/ajas.18.0256.
    [14]
    BICHSEL K J, GOGIA N, MALOUFF T, et al. Role for the epidermal growth factor receptor in chemotherapy-induced alopecia [J]. PLoS ONE, 2013, 8(7): e69368.
    [15]
    PAIK S H, YOON J S, RYU H H, et al. Pretreatment of epidermal growth factor promotes primary hair recovery via the dystrophic anagen pathway after chemotherapy-induced alopecia[J]. Experimental Dermatology, 2013, 22(7): 496-499.
    [16]
    WEE P, WANG Z. Epidermal growth factor receptor cell proliferation signaling pathways [J]. Cancers, 2017, 9(5): 52.
    [17]
    HUYNH E, LI J. Generation of Lactococcus lactis capable of coexpressing epidermal growth factor and trefoil factor to enhance in vitro wound healing [J]. Applied Microbiology and Biotechnology, 2015, 99(11): 4667-4677.
    [18]
    WALTER M N, WRIGHT K T, FULLER H R, et al. Mesenchymal stem cell-conditioned medium accelerates skin wound healing: An in vitro study of fibroblast and keratinocyte scratch assays [J]. Experimental Cell Research, 2010, 316(7): 1271-1281.
    [19]
    LUSK J B, LAM V Y, TOLWINSKI N S. Epidermal growth factor pathway signaling in drosophila embryogenesis: Tools for understanding cancer [J]. Cancers, 2017, 9(2): 16.
    [20]
    XING X J, YANG L, YOU Y, et al. Study of the biological function and penetration pathways of the mouse epidermal growth factor ethosomal delivery system [J]. Experimental Dermatology, 2011, 20(11): 945-947.
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